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| 基于纳米多孔硅Bragg反射镜免标记生物传感器的研究 | |
| 引用本文： | 赵晶,宋诗莹,张子怡,傅晶晶,曾志鹏,张娟琨. 基于纳米多孔硅Bragg反射镜免标记生物传感器的研究[J]. 生物加工过程, 2010, 8(5): 59-63. DOI: 10.3969/j.issn.1672-3678.2010.05.013 |
| 作者姓名： | 赵晶 宋诗莹 张子怡 傅晶晶 曾志鹏 张娟琨 |
| 作者单位： | 1. 天津科技大学,生物工程学院,工业微生物教育部重点实验室,天津市工业微生物重点实验室,天津,300457 2. 天津科技大学,现代分析技术研究中心,天津,300457 3. 天津体育学院,运动人体科学系,天津,300381 |
| 基金项目： | 科技部资助项目，天津市科委资助项目 |
| 摘 要： | 构建一种基于多孔硅Bragg反光镜的免标记的纳米生物传感器,通过在纳米多孔硅上固定的适配子的特异性识别能力,检测溶液中四环素浓度。当适配子结合不同浓度的四环素溶液时,引起多孔硅Bragg反光镜内部有效折射率的变化,反射谱峰位进而发生变化。传感器的有效检测范围为1~200μg/L,检测限为1μg/L。 |
| 关 键 词： | 免标记生物传感器 多孔硅Bragg反射镜 反射光谱 |
Marker-free biosensor based on nano-porous silicon Bragg reflector |
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| ZHAO Jing,SONG Shi-ying,ZHANG Zi-yi,FU Jing-jing,ZENG Zhi-peng,ZHANG Juan-kun. Marker-free biosensor based on nano-porous silicon Bragg reflector[J]. Chinese Journal of Bioprocess Engineering, 2010, 8(5): 59-63. DOI: 10.3969/j.issn.1672-3678.2010.05.013 | |
| Authors: | ZHAO Jing SONG Shi-ying ZHANG Zi-yi FU Jing-jing ZENG Zhi-peng ZHANG Juan-kun |
| Affiliation: | 1.College of Bioengineering,Key Laboratory of Industrial Microbiology of Ministry of Education,and Key Laboratory of IndustrialMicrobiology of Tianjin,Tianjin University of Science and Technology,Tianjin 300457,China;2.Modern Analysis TechnologyResearch Center,Tianjin University of Science and Technology,Tianjin 300457,China;3.Department of Health and ExerciseScience,Tianjin University of Sports,Tianjin 300381,China) |
| Abstract: | A kind of marker-free nano-biosensor based on the porous silicon Bragg reflector was constructed for detecting the tetracycline specifically in the solution with the capability of the nano-porous silicon-fixed aptamer.When the aptamer was integrated with tetracycline solution of different concentrations,changes would occur by the effective refractive index within the porous silicon Bragg reflector,thus it would further generate changes with the reflection spectrum peaks.The effective detection range of the sensor was 1 μg/L to 200 μg/L and the detection limit was 1 μg/L. |
| Keywords: | marker-free biosensor porous silicon Bragg reflector reflectance spectrum |
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