Role of epidermal stem cells in repair of partial-thickness burn injury after using Moist Exposed Burn Ointment (MEBO) histological and immunohistochemical study |
| |
Authors: | M.R. EL-Hadidy A.R. EL-Hadidy A. Bhaa S.A. Asker S.A. Mazroa |
| |
Affiliation: | 1. Plastic and Reconstructive Surgery Department, Faculty of Medicine, Mansoura University, Egypt;2. Histology & Cell Biology Department, Faculty of Medicine, Mansoura University, Egypt |
| |
Abstract: | Moist Exposed Burn Ointment (MEBO®) is widely used topical agent applied on skin burn. This study investigated the effect of MEBO topical application on activation and proliferation of epidermal stem cells through the immunohistochemical localization of cytokeratin 19 (CK19) as a known marker expressed in epidermal stem cells. Biopsies from normal skin and burn wounds were taken from 21 patients with partial thickness burn 1, 4, 7, 14, 21, and 28 days after treatment with MEBO. Tissue sections were prepared for histological study and for CK19 immunohistochemical localization. In control skin, only few cells showed a positive CK19 immune-reaction. Burned skin showed necrosis of full thickness epidermis that extended to dermis. Gradual regeneration of skin accompanied with an enhancement in CK19 immune-reactivity was noted 4, 7, 14 and 21 days after treatment with MEBO. On day 28, a complete regeneration of skin was observed with a return of CK19 immune-reactivity to the basal pattern again. In conclusion, the enhancement of epidermal stem cell marker CK19 after treatment of partial thickness burn injuries with MEBO suggested the role of MEBO in promoting epidermal stem cell activation and proliferation during burn wound healing. |
| |
Keywords: | MEBO, Moist Exposed Burn Ointment CK19, cytokeratin 19 UAE, United Arab Emirates USA, United States of America LAB, labeled avidin&ndash biotin PBS, phosphate buffered saline DAB, 3,3&prime -diaminobenzidine SD, standard deviation H& E, hematoxylin and eosin IHC, immunohistochemistry |
本文献已被 ScienceDirect 等数据库收录! |
|