Establishment of recipient model for spermatogonial stem cells transplantation in Kunming mice |
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| Authors: | Juhua Wang Xiuheng Xue Caiyun Fan Yunsheng Li Yong Pu Hongguo Cao Xiaorong Zhang |
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| Institution: | 1. College of Animal Science and Technology, Anhui Agricultural University, Hefei, Anhui 230036, China;2. Anhui Provincial Laboratory of Local Livestock and Poultry Genetical Resource Conservation and Breeding, Hefei, Anhui 230036, China |
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| Abstract: | The objective of this study was to establish a recipient model for spermatogonial stem cells (SSCs) transplantation in the Kunming mice after different doses busulfan treatment. The results showed that the most optimal dose of busulfan was 20 mg/kg and the most appropriate time for transplantation was 5–7 wk after busulfan treatment. Then, the cloned fragments existed in the testis of recipient mice after 20 mg/kg busulfan treatment and the offspring with enhanced green fluorescent protein (EGFP) were produced by the transplanting SSCs. Hence, we established the effective recipient model for donor-derived SSCs transplantation in Kunming mice. |
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| Keywords: | Busulfan Kunming mice Spermatogonial stem cells Testis Transplantation |
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