| 电穿孔技术在转基因及动物克隆中的应用 |
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| 引用本文： | 郝新保,常智杰. 电穿孔技术在转基因及动物克隆中的应用[J]. 生物技术通报, 2003, 0(1): 1-3,7 |
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| 作者姓名： | 郝新保 常智杰 |
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| 作者单位： | 1. 清华大学生物系基因组研究所,北京,100084;第四军医大学全军骨肿瘤研究所,西安,710038
2. 清华大学生物系基因组研究所,北京,100084 |
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| 摘 要： | 电穿孔技术利用电场造成细胞膜的改变而将DNA导入细胞内，它还可用于细胞融合及动物克隆等。基因电转移的效率通常比化学法提高1—2个数量级，主要与脉冲波形、长度、缓冲液等有关。方波直流电脉冲应用广泛，在有关细胞核移植的多项研究报告中均指出它有重要作用。
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| 关 键 词： | 电穿孔技术 转基因 动物克隆 应用 胚胎克隆 细胞融合 细胞核移植 |
Application of Electroporation in Gene Transfer and Animal Embryo Cloning |
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| Hao Xinbao , Chang Zhijie. Application of Electroporation in Gene Transfer and Animal Embryo Cloning[J]. Biotechnology Bulletin, 2003, 0(1): 1-3,7 |
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| Authors: | Hao Xinbao Chang Zhijie |
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| Affiliation: | Hao Xinbao 1,2 Chang Zhijie1 |
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| Abstract: | Electroporation can make gene transfer into cells, cell fusion and in vitro fetilization through electric field on cells. The gene transfer efficiency of electroporation is usually ten to hundreds times higher than chemical methods, which is mostly associated with the pulse shape, length and the buffers. The DC square pulse is widely used in animal embryo cloning and nuclear transfer. |
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| Keywords: | Gene transfer Animal embryo cloning Electroporation |
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