| Abstract: | The CM-proteins, which are salt-soluble proteins that can beextracted with chloroform: methanol (2: 1, v/v), seem to bepresent in the endosperm of all the cereal species investigated.Antibodies raised against a mixture of the barley CM-proteins(A-H) cross-reacted with wheat and rye proteins in Ouchterlonytests and a detailed study was carried out for purified wheat(CM1, CM2. CM3. CM3') and barley (CMa, CMb, CMc, CMd) CM-proteins. 35Sl-Cysteine-labelled endosperm proteins from wheat and barleywere investigated by immuno-precipitation, electrophoresis andfluorography using the antibodies (A-H) and also those to amixture of wheat CM-proteins and to CMd. There was completeantigenic identity for all the wheat proteins and CMd, someof the other proteins showed partial antigenic identity. Previously proposed genetic and biochemical relationships amongthese proteins were confirmed in the present study. Key words: CM-Protein, Cereal endosperm, Immunochemistry |
