Nanoparticles for the delivery of genes and drugs to human hepatocytes |
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Authors: | Yamada Tadanori Iwasaki Yasushi Tada Hiroko Iwabuki Hidehiko Chuah Marinee K L VandenDriessche Thierry Fukuda Hideki Kondo Akihiko Ueda Masakazu Seno Masaharu Tanizawa Katsuyuki Kuroda Shun'ichi |
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Institution: | Institute of Scientific and Industrial Research, Osaka University, 8-1 Mihogaoka, Ibaraki, Osaka 567-0047, Japan. |
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Abstract: | Hepatitis B virus envelope L particles form hollow nanoparticles displaying a peptide that is indispensable for liver-specific infection by hepatitis B virus in humans. Here we demonstrate the use of L particles for the efficient and specific transfer of a gene or drug into human hepatocytes both in culture and in a mouse xenograft model. In this model, intravenous injection of L particles carrying the gene for green fluorescent protein (GFP) or a fluorescent dye resulted in observable fluorescence only in human hepatocellular carcinomas but not in other human carcinomas or in mouse tissues. When the gene encoding human clotting factor IX was transferred into the xenograft model using L particles, factor IX was produced at levels relevant to the treatment of hemophilia B. The yeast-derived L particle is free of viral genomes, highly specific to human liver cells and able to accommodate drugs as well as genes. These advantages should facilitate targeted delivery of genes and drugs to the human liver. |
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