应用ITS序列分析探讨偏花报春的系统位置

对于偏花报春Primula secundiflora Franch. 的系统位置，主要有两种意见，一种认为偏花报春具有典型的钟状花冠，应置于钟花报春组Sect．Sikkimensis；而另一种意见则依据其他特征将其置于灯台报春组sect．Proliferae。通过对偏花报春、灯台报春组4种植物和钟花报春组3种植物核糖体DNA中的内转录间隔区(ITS)的序列测定及分析，并结合形态学及染色体特征的比较论证，认为偏花报春应置于灯台报春组。

Systematic position of Primula secundiflora (Primulaceae) inferred from nuclear ribosomal DNA ITS sequence data

Two views on the systematic position of Primula secundiflora Franch.were presented by different scholars. This species has long been treated as a member of sect. Sikkimensis, owing to its campanulate corolla, but recently it was moved to sect. Proliferae based on other characteristics emphasized. In the present paper, the ITS (internal transcribed spacer) regions of nuclear ribosomal DNA from eight Primula species (three in sect. Sikkimensis, four in sect. Proliferae together with P. secundiflora) were sequenced and analyzed. Based on the results,together with evidence from morphology and cytology, we consider that P. secundiflora should be placed in sect. Proliferae.