Isolation and characterization of chemically transformed pancreatic acinar cell lines from young and old mice |
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Authors: | Karen Zelinsky-Papez Timothy H Carter Jay A Zimmerman |
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Institution: | (1) Department of Biological Sciences, St. John’s University, 11439 New York, New York |
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Abstract: | Summary To evaluate the role of animal age in chemically induced transformation, pancreatic cells were grown in culture 6 to 8 wk
after injecting mice at either 6 or 22 mo. of age with a single dose ofN-methyl-N-nitrosourea (NMU). The cell type and the frequency with which lines were obtained from aged animals paralleled the frequency
and pattern of tumor induction by NMU in vivo. Outgrowth of pancreatic explants from young animals required the presence of
the tumor promoter 12-o-tetradecanoyl-phorbol-13-acetate to establish continuously growing cell lines. Whereas NMU alone produced
lines from aged mice, the promoter did not increase the frequency with which continuous lines were recovered from the aged
animals. Of eight cloned cell lines (four young and four old), all had characteristics of transformed mouse pancreatic acinar
cells when tested for lectin binding, lactate dehydrogenase isozyme pattern, chromosome number, and anchorage-independent
growth. Cell lines derived from aged animals were slower growing and had higher chromosome numbers than lines derived from
their younger counterparts. |
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Keywords: | pancreatic carcinoma acinar cells aging carcinogenesis 12-o-tetradecanoyl-phorbol-13-acetate |
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