| 庆大霉素产生菌原生质体融合高产株与发酵罐试产的研究 |
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| 引用本文： | 管玉霞,蓝基贤,严广兴,郑景世.庆大霉素产生菌原生质体融合高产株与发酵罐试产的研究[J].中国生物工程杂志,2009,29(8):62-67. |
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| 作者姓名： | 管玉霞 蓝基贤 严广兴 郑景世 |
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| 作者单位： | 福州大学生物科学与工程学院 福州 350002 |
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| 摘 要： | 对庆大霉素产生菌绛红色小单孢菌（Micromonospora purpurea）原生质体分别用硫酸二乙酯（DES）和紫外线（uv）进行诱变、融合，用庆大霉素进行抗性筛选、再生后得到高产菌株；经摇瓶连续10批次发酵考查，平均发酵单位在2200±U/ml；又经5L发酵罐连续发酵考查7批次，产量平均1900±U/ml。产品质量符合药典。
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| 关 键 词： | 绛红色小单孢菌 原生质体诱变融合 抗性筛选 发酵罐产量 |
| 收稿时间： | 2009-01-20 |
| 修稿时间： | 2009-05-12 |
Study on the High Yield Strain of Gentamicin Producer by Protoplast Fused and Fermentor Test |
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| GUAN Yu-xia,LAN Ji-xian,YAN Guang-xing,ZHENG Jing-shi.Study on the High Yield Strain of Gentamicin Producer by Protoplast Fused and Fermentor Test[J].China Biotechnology,2009,29(8):62-67. |
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| Authors: | GUAN Yu-xia LAN Ji-xian YAN Guang-xing ZHENG Jing-shi |
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| Institution: | College of Biological Science and Technology, Fuzhou University, Fuzhou350002,China |
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| Abstract: | The protoplasts of Micromonospora purpurea, the high yield strains of gentammicin producer were mutagenized by diethyl sulfate (DES) and ultraviolet radiation (UV)respectively， then fused, screened by gentamicin resistance and regenerated. The average fermentation unit 2200±U/ml could be achieved by shake flask for 10 batches. The average fermentation unit 1900±U/ml could be obtained by 5L fermentor for 7 batches. The quality of the end product conformed to CP2000, BP2000 and USP26 pharmacopoeia. |
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| Keywords: | Micromonospora purpurea protoplast mutagenesis fusion screened resistance yield of fermentor |
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