Fluorescence immunolocalization of bound atrazine residues in plant tissue |
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Authors: | Gabriele Sohn Christof Sautter Bertold Hock |
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Institution: | 1. School of Biological Sciences, University College of North Wales, LL57 2UW, Bangor, Gwynedd, UK
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Abstract: | Bound atrazine was detected inElodea canadensis by an improved immunohistochemical fluorescence procedure using anti-triazine antibodies from rabbits, biotin-labelled anti-rabbit
immunoglobulin G and streptavidin-phycoerythrin conjugate. Whereas no labelling was found in control plants grown in charcoal-filtered,
atrazine-free water, the labelling of plants obtained from their natural habitat and grown in tap water was sometimes nearly
as high as in samples loaded with atrazine. The efficiency of the immunofluorescence procedure was compared using several
antisera obtained by immunizing with different hapten conjugates and purified by various purification methods. The best results
were observed with the atrazine analogue ametryn sulfoxide, which was coupled to bovine serum albumin for immunization and
to Sepharose for immunoaffinity chromatography. The procedure described in this paper may serve as a general tool for detecting
bound pesticide residues in plant material.
Dedicated to Professor Hans Mohr on the occasion of his 60th birthday |
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Keywords: | Atrazine (immunolocalization) Elodea Herbicide (bound residues) Phycoerythrin |
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