Seasonal dynamic changes in bacterial compositions in the Inner Mongolia desert steppe |
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Authors: | Ping Lu Wanli Ma Hao Li Yongsheng Wu Guifen Lv Jizheng He |
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Affiliation: | (1) College of Life Science and Technology, Inner Mongolia Normal University, Huhhot, 010022, China;(2) Research Center of Ecology and Environment, Chinese Academy of Sciences, Beijing, 100083, China;(3) Planning and Design Academy of Forestry Products Industry, State Forestry Administration, Beijing, 100714, China |
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Abstract: | Polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) technique was employed to examine the seasonal dynamic changes in bacterial community composition in the Inner Mongolia desert steppe using specific primers F954 and R1369. Bright and reproducible bands were sequenced, and the phylogenic tree was constructed. The results show that the bacterial community composition changed between different seasons. The specific bands were different between the sampling sites with light and heavy levels of degraded grassland. Three main types of bacteria constituting the microbial community in the Inner Mongolia desert steppe belonged to the α, γ andgd-sub phyla of Proteobacteria, Bacteroidetes and Acidobacteria. The unculturable bacteria accounted for 69% of the whole bacterial community of the Inner Mongolia desert steppe. |
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Keywords: | Polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) Inner Mongolia desert steppe community composition seasonal dynamic change |
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