In vitro culture of immature embryos of Howea forsteriana Becc. |
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Authors: | Isabel Moura Maria Filomena Neves Carneiro |
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Affiliation: | (1) Jardim-Museu Agrícola Tropical, Calçada do Galvão, 1400 Lisboa;(2) Centro de Investigação das Ferrugens do Cafeeiro, 2780 Oeiras, Portugal |
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Abstract: | Immature zygotic embryos from Howea forsteriana Becc. were cultured on the Murashige and Skoog medium, supplemented with myo-inositol, thiamine-HCl and activated charcoal, in the absence of growth regulators. The fruits were stored for 4 weeks at +4°C and at –18°C. The excised embryos from the fruits stored at +4°C developed into plantlets, showing a well developed primary root, after 40 days in culture, while those excised from fruits stored at –18°C exhibited no growth.This is the first time that in vitro culture and plantlet regeneration from immature embryos of Howea forsteriana has been obtained. |
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Keywords: | embryo culture Howea forsteriana propagation palms |
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