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Construction of a bifunctional genetically labelled plasmid for Bacillus thuringiensis subsp. israelensis
Authors:Vanya I Miteva  Rosa T Grigorova
Institution:(1) Department of Genetics, Institute of Microbiology, Bulgarian Academy of Sciences, BG-1113 Sofia, Bulgaria
Abstract:A small cryptic plasmid of Bacillus thuringiensis subsp. israelensis was labelled in vitro with two genetic markers. One of the recombinant plasmids was mapped and transformed in Escherichia coli, Bacillus subtilis and Bacillus thuringiensis. This and similar shuttle plasmids could be very useful as vectors for the investigation of the toxin genes in their own host.Abbreviations BTI Bacillus thuringiensis subsp. israelensis - MDal megadaltons
Keywords:Bacillus thuringiensis subsp  israelensis  Shuttle plasmid  Construction in vivo  Escherichia coli  Bacillus maintenance
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