Construction of a bifunctional genetically labelled plasmid for Bacillus thuringiensis subsp. israelensis |
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Authors: | Vanya I Miteva Rosa T Grigorova |
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Institution: | (1) Department of Genetics, Institute of Microbiology, Bulgarian Academy of Sciences, BG-1113 Sofia, Bulgaria |
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Abstract: | A small cryptic plasmid of Bacillus thuringiensis subsp. israelensis was labelled in vitro with two genetic markers. One of the recombinant plasmids was mapped and transformed in Escherichia coli, Bacillus subtilis and Bacillus thuringiensis. This and similar shuttle plasmids could be very useful as vectors for the investigation of the toxin genes in their own host.Abbreviations BTI
Bacillus thuringiensis subsp. israelensis
- MDal
megadaltons |
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Keywords: | Bacillus thuringiensis subsp israelensis Shuttle plasmid Construction in vivo Escherichia coli Bacillus maintenance |
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