Phenotypic character of the lipid hydroperoxide-resistant mutant: Positive relationship between flocculation and resistance against lipid hydroperoxide inSaccharomyces cerevisiae |
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Authors: | Y. Inoue L. -T. Tran K. Yoshikawa A. Kimura |
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Affiliation: | (1) Research Institute for Food Science, Kyoto University, 611 Uji, Kyoto, Japan |
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Abstract: | A lipid hydroperoxide-resistant mutant was isolated from a strain ofSaccharomyces cerevisiae. The mutant was resistant to 1.5mmtert-butylhydroperoxide and 1.0mm linoleic acid hydroperoxide. It flocculated in a Ca2+-dependent manner and the resistance against lipid hydroperoxide was suppressed by mannose, which also inhibited flocculation. A positive relationship between the acquirement of, the flocculent phenotype and resistance against lipid hydroperoxide is suggested. A protein with a molecular weight of 33 kDa was found on the surface of the mutant cell. |
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Keywords: | Flocculation linoleic acid hydroperoxide lipid hydroperoxide Saccharomyces cerevisiae |
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