Clydonella sawyeri n. sp. (Amoebozoa,Vannellida): Morphological and molecular study and a re-definition of the genus Clydonella Sawyer, 1975 |
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| Affiliation: | 1. Department of Invertebrate Zoology, Faculty of Biology, St. Petersburg State University, Universitetskaya nab. 7/9, 199034 St. Petersburg, Russia;2. Laboratory of Parasitic Worms and Protistology, Zoological Institute RAS, Universitetskaya nab. 1, 199034 St. Petersburg, Russia;1. Department of Invertebrate Zoology, Faculty of Biology, St. Petersburg State University, St. Petersburg, Russia;2. Penard Labs, Cape Town, South Africa;3. Laboratory of Cellular and Molecular Protistology, Zoological Institute, Russian Academy of Science, St. Petersburg, Russia;1. Papanin Institute for Biology of Inland Waters Russian Academy of Science, Borok 152742, Russia;2. Laboratory of Cellular and Molecular Protistology, Zoological Institute Russian Academy of Science, Saint Petersburg 199034, Russia;3. Department of Botany, University of British Columbia, Vancouver, V6T 1Z4, British Columbia, Canada;1. Department of Invertebrate Zoology, Faculty of Biology, Saint-Petersburg State University, 199034 Saint-Petersburg, Russia;2. Irkutsk Antiplague Research Institute of Siberia and Far East, 664047 Irkutsk, Russia |
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| Abstract: | We isolated and described a brackish-water amoeba, Clydonella sawyeri n. sp. (Amoebozoa, Vannellida), from the littoral habitat in Kandalaksha Bay (The White Sea, northwestern Russia). Morphology of this amoeba corresponds to the initially proposed diagnosis of the genus Clydonella Sawyer, 1975, although it is sufficiently different from other described species of this genus to warrant a distinct species designation. Phylogenetic analysis based on the small-subunit rRNA gene shows that this species is closely related to the two previously studied ATCC strains identified as Clydonella sp., for which only ultrastructural and molecular data were published. Cytochrome oxidase subunit 1 (COI) gene sequence of the studied species was for the first time obtained for Clydonella. Analysis of this marker shows that this genus belongs to Vannellida and is separated from the other vannellids sampled to date. Phylogenetic analysis of the concatenated SSU rRNA and COI genes dataset yields the best resolved position of Clydonella compared to both markers when analysed separately. Based on the data presented we finally link light microscopic, ultrastructural and molecular data in a description of a single strain, which allows a refinement of the diagnosis of the genus Clydonella. |
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| Keywords: | Amoebae Light microscopy Molecular phylogeny Taxonomy Ultrastructure |
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