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Whole genome amplification from plant cell colonies of somatic hybrids using strand displacement amplification |
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| Authors: | S. V. Raikar C. Bryant R. Braun A. J. Conner M. C. Christey |
| Affiliation: | (1) National Centre for Advanced Bio-Protection Technologies, Lincoln University, PO Box 84, Canterbury, New Zealand;(2) New Zealand Institute for Crop & Food Research Limited, Private Bag 4704, Christchurch, New Zealand;(3) Pastoral Genomics, PO Box 109-185, Newmarket, Auckland, New Zealand |
| Abstract: | The application of strand displacement amplification (SDA) is demonstrated for whole genome amplification from nanograms to micrograms for DNA isolated from small plant cell colonies. Secondary digest amplified fragment length polymorphism (SD-AFLP) analysis confirmed that the amplified genome is a representative of the entire genome. This approach allows the amplification of DNA isolated from small cell colonies of putative somatic hybrids for rapid molecular confirmation of the hybrid status of fusion products. |
| Keywords: | Intergeneric Lotus– Lolium Protoplast fusion Strand displacement amplification Whole genome amplification |
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