A 5′-amino analog of adenosine diphosphate

The synthesis and properties of the 5′-amino analog of adenosine diphosphate are described. The 5′-amino-5′-deoxyadenosine 5′-N-diphosphate (_NADP) is prepared from the previously described aminonucleoside triphosphate by the hexokinase catalyzed transfer of the terminal phosphoryl to glucose. The _NADP is stable in neutral or basic media, and is similar to natural ADP in chromatographic, electrophoretic, and spectrographic properties. Snake venom phosphodiesterase degrades _NADP to the monophosphate _NAMP, and acid degrades both _NADP and _NAMP to 5′-amino-5′-deoxyadenosine.