Studies of prostaglandins and prostaglandin antagonists on bovine pulmonary vein in vitro |
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Authors: | J.F. Burka P. Eyre |
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Affiliation: | Department of Biomedical Sciences University of Guelph Guelph, Ontario, Canada, N1G 2W1 |
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Abstract: | Acetylsalicylic acid (ASA), indomethacin, sodium meclofenamate (FEN), phenylbutazone (PB), phloretin phosphates (PP), SC-19220, and diethylcarbamazine citrate (DECC) were screened against histamine, 5-hydroxytryptamine (5-HT), bradykinin, acetylcholine, and prostaglandins (PG) E1, E2, and F2α to determine their specificity in antagonizing PG's on the bovine pulmonary vein. PG E2 relaxed the smooth muscle preparation at low concentrations and induced contraction at higher concentrations. PG E1 consistently evoked dose-related relaxations, whereas PG F2α contracted the bovine pulmonary vein. Studies with inhibitors suggest that the different actions of prostaglandins could be mediated through different receptors. Sodium meclofenamate and PP dimer blocked PG E2-induced contractions, whereas relaxations were not blocked. DECC inhibited the relaxant effect of PG E2. DECC also antagonized histamine, 5-HT, and PG F2α, suggesting the drug is rather non-specific. Phenylbutazone antagonized the actions of both PG E2 and PG F2α on the bovine pulmonary vein. By classifying receptors by antagonism the bovine pulmonary vein appears to contain PG E2 (PP-type), PG E2 (FEN-type), PG E2 (PB-type), and PG F2α (PB-type) receptors. An absence of SC-type PG-receptors is noted. |
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