Comparison between the effect of luteinizing hormone and prostaglandin E1 on ovarian cyclic AMP |
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Authors: | Gunnar Selstam Jan Liljekvist Sten Rosberg Lena Grönquist Torsten Perklev Kurt Ahrén |
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Institution: | Department of Physiology, University of Göteborg, Göteborg, and Research Laboratories, AB Leo, Helsingborg, Sweden |
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Abstract: | Isolated whole ovaries from 23–24 day-old rats were studied in order to compare the effects of prostaglandin E1 (PGE1) and luteinizing hormone (LH) on ovarian cyclic adenosine 3′,5′-monophosphate (cAMP) production. Both substances produced a dose-dependent accumulation of cAMP in the ovarian tissue as well as in the incubation medium. The release of cAMP to the incubation medium was considerable after long periods of incubation (60–120 min). Time-relationships for LH- and PGE1-effects were different. Maximal cAMP content in the tissue after addition of PGE1 was seen already after 5–15 min of incubation whereas LH gave a maximal response after around 60 min. Accumulation of cAMP in the medium was approximately linear with time for both LH and PGE1. Addition of theophylline potentiated the action of PGE1 and LH but did not change the time-courses of the effects. It is concluded that the accumulation of cAMP in the medium should be considered in studies with various in vitro types of ovarian preparations. It is also pointed out that the different time-courses of the LH- and PGE1-effects make the interpretation of additivity experiments difficult. |
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