Enzyme-catalyzed free radical reactions with nicotinamide-adenine nucleotides: I. Lactate dehydrogenase-catalyzed chain oxidation of bound NADH by superoxide radicals |
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| Authors: | Benon HJ Bielski Phillip C Chan |
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| Institution: | Chemistry Department, Brookhaven National Laboratory, Upton, New York 11973 U.S.A.;Department of Biochemistry, State University of New York Downstate Medical Center, Brooklyn, New York, 11203 U.S.A. |
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| Abstract: | The radiation-induced oxidation of NADH by Superoxide radicals proceeds in the presence of lactate dehydrogenase by a chain mechanism. The reaction is initiated by O2? radicals, and propagated by O2. The chain length is a function of NADH]/lactate dehydrogenase], the concentration of O2, the dose rate, and pH. The chain reaction can be inhibited by addition of ascorbic acid. |
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