Studies on invitro DNA synthesis: II. Isolation of a protein which stimulates deoxynucleotide incorporation catalyzed by DNA polymerases of E.coli |
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Authors: | Jerard Hurwitz Sue Wickner Michel Wright |
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Institution: | Department of Developmental Biology and Cancer Albert Einstein College of Medicine Bronx, New York 10461 USA |
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Abstract: | Purified RNA polymerase, DNA polymerase III and unwinding protein of catalyze limited rifampicin sensitive fd or ØX 174 DNA-dependent DNA synthesis. A protein has been partially purified from which stimulates rifampicin sensitive dXMP incorporation in this system 20 to 30 fold. This protein also stimulates DNA synthesis catalyzed by DNA polymerases I and II; the stimulation occurs in reactions primed with natural and synthetic DNAs as well as RNA-DNA hybrids. The protein is not a product of the known dna genes. In contrast to the above system of purified enzymes, rifampicin sensitive dXMP incorporation in crude extracts of is specifically dependent on fd but not ØX 174 DNA. An additional factor has been isolated from extracts of which restores specificity to the purified rifampicin sensitive system by preventing ØX 174 DNA from serving as a template. |
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