Ouabain binding sites and the (Na+,K+)-ATPase of brain microsomal membranes |
| |
Authors: | W.E. Harris P.D. Swanson W.L. Stahl |
| |
Affiliation: | Division of Neurology and the Department of Physiology and Biophysics, the University of Washington School of Medicine and the Neurochemistry Laboratory, Veterans Administration Hospital, Seattle, Wash. U.S.A. |
| |
Abstract: | Beef brain microsomes bound approximately 180–220 pmoles of [3H]ouabain per mg of protein in the presence of either MgCl2 and inorganic phosphate or ATP, MgCl2 and NaCl. The ouabain-binding capacity and the ouabain-membrane complex were more stable than the (Na+,K+)-ATPase activity to treatment with agents known to affect the membrane integrity, such as, NaClO4, sodium dodecyl sulfate, , urea. ultrasonication, heating, pH and phospholinase C.The presence of binding sites that were normally inaccessible to ouabain in brain microsomes was demonstrated. These sites appeared after disruption of microsomes with 2 M NaClO4 as evidenced by increased binding of [3H]ouabain. These sites may be buried during the subcellular fractionation procedure and could be accessible in the intact cell. |
| |
Keywords: | PCMB Reprint requests to: Neurochemistry Laboratory, Veterans Administration Hospital, Seattle, Wash. 98108, U.S.A. |
本文献已被 ScienceDirect 等数据库收录! |
|