Department of Chemistry University of Massachusetts Amherst, Massachusetts 01002 USA
Abstract:
Single crystals of horse CoHb were obtained by reduction of CoHb+ crystals with dithionite. Epr measurements showed that the g? and Coà tensors are both axial and share the same principal axis systems. Of the four subunits, the “heme” normals of C? and d? subunits ãb?plane 29 ± 1° from b?; they have the same orientation as the hemes in methemoglobin. The normals of “hemes” à and B? are 47 above the ãb? plane as compared to 16° in methemoglobin.