New results on the mode of action of 3,-(3,4-dichlorophenyl)-1,1-dimethylurea in spinach chloroplasts |
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Authors: | Pierre Bennoun Yung-sing Li |
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Institution: | Institut de Biologie Physico-Chimique, Paris 5e France |
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Abstract: | Simultaneous measurements of hydroxylamine photo-oxidation and fluorescence induction were performed in the presence of 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU). The results provide a justification for the common use of fluorescence data to estimate the concentration of active System II centers in the presence of inhibitors.The addition of DCMU to dark-adapted chloroplasts under special conditions induces a large increase of the initial yield of fluorescence. A reversible inactivation of part of the System II centers is responsible for this effect. Similar data were obtained with other classical inhibitors of oxygen evolution. |
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Keywords: | DCMU 3-(3 4-dichlorophenyl)-1 1-dimethylurea |
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