Properties of ATPase of gastric mucosa V. Preparation of membranes and mitochondria by zonal centrifugation |
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| Authors: | J.G. Spenney A. Strych A.H. Price H.F. Helander G. Sachs |
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| Affiliation: | 1. University of Alabama in Birmingham, Birmingham, Ala. U.S.A.;2. University of Umeå, Umeå Sweden |
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| Abstract: | Using zonal sucrose density gradient centrifugation methods to fractionate the subcellular components in gastric mucosal homogenates have been developed. Methods are described which give high yield or rapidity in fractionation. A procedure is described which enables large-scale preparations of smooth walled vesicular membranes containing HCO3?-ATPase activity free from mitochondrial contamination as assessed by electron microscopic morphology and undetectable succinic dehydrogenase or monoamine oxidase activity. A method to purify gastric mitochondria is also described. |
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| Keywords: | To whom correspondence should be sent. |
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