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Effect of gibberellic acid on mevalonate activation in germinating Corylus avellana seeds
Authors:Peter R. Shewry  Neville J. Pinfield  Allan K. Stobart
Affiliation:Plant Cell Metabolism Laboratory, Department of Botany, Bristol University, Bristol BS8 1UG U.S.A.
Abstract:Giberellic acid (GA) induced germination of hazel seeds is accompanied by early increases in the specific and total activities of MVA kinase in the embryonic axes. This is followed by an increase in the activity of decarboxylation of MVA by the whole axes. The activity of MVA kinase in the cotyledons is not affected by GA treatment although increased uptake of MVA results in increased decarboxylation by cotyledon slices. The effects of cofactors and inhibitors on the activities of MVA kinase and MVA decarboxylation in a cell free extract of hazel cotyledons are described.
Keywords:Betulaceae  hazel seeds  mevalonic acid kinase  MVA-decarboxylation  gibberellic acid.  MVA-P—mevalonic acid phosphate  MVA-PP—mevalonic acid pyrophosphate  IPP—isopentenyl pyrophosphate  IPP—isopentenyl pyrophosphate  HMGCoA—β-hydroxy β methyl glutaryl CoA  NEM—N-ethyl maleimide.
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