Chlorophyll a forms in Phaeodactylum tricornutum: Comparison with other diatoms and brown algae |
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Authors: | JC Goedheer |
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Institution: | Biophysical Research Group, Institute of Physics, State University, Utrecht The Netherlands |
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Abstract: | The long-wave chlorophyll a forms in Phaeodactylum tricornutum (688 and 703 nm) change into a short-wave form, 670 nm, as a result of incubation with 55% glycerol, freeze-thawing, short ultraviolet irradiation and, probably, chloroplast preparation. This short-wave form is non-fluorescent. Fluorescence polarisation measurements indicate that the long-wave chlorophyll a molecules are oriented parallel to each other. Although “labile” long-wave chlorophyll a receives energy from Photosystem II pigments at room temperatures and follows the induction phenomena of fluorescence, it is indicated by afterglow experiments that it probably does not participate in Photosystem II.Long-wave chlorophyll forms in Fucus are stable and probably are related to Photosystem I. |
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Keywords: | DCMU 3-(3 4-dichlorophenyl)-1 1-dimethylurea |
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