A kinetic assay of TPNH-dependent microsomal lipid peroxidation by changes in difference spectra |
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| Authors: | Hubert E May Donald J Reed |
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| Institution: | Department of Natural Science, Oral Roberts University, Tulsa, Oklahoma 74105 USA;Department of Biochemistry and Biophysics, Oregon State University, Corvallis, Oregon, 97331 USA |
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| Abstract: | In the presence of TPNH, O2 and ADP-Fe+3 rat liver microsomes yield difference spectral changes at 237 nm and 267–270 nm that correlate with the kinetics of lipid peroxidation as measured by the rate of malonaldehyde formation and O2 and TPNH consumption. Mn+2 EDTA, aniline, and reduced glutathione were inhibitory. It is suggested that the difference spectral changes at 237 nm and 267–270 nm are essentially due to conjugated diene and malonaldehyde formation, respectively. |
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