Further studies on the cell-free synthesis of procollagen-collagen by chick embryo polysomes |
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| Authors: | Thomas M. Burns Carlos L. Spears S.S. Kerwar |
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| Affiliation: | Roche Institute of Molecular Biology, Nutley, NJ 07110 U.S.A. |
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| Abstract: | Free and membrane bound polysomes were prepared from 8-day-old chick embryos. Both polysome preparations were equally active in protein synthesis but procollagen-collagen synthesis was carried out exclusively by the membrane bound polysomes. The collagenous product was analyzed by DEAE-cellulose chromatography and after hydroxylation with peptidyl proline hydroxylase had a hydroxyproline/proline ratio of 0.77. This suggests that the collagenous product synthesized by the membrane bound polysomes is procollagen. |
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