Genetic transformation in : The inhibitory role of the DNase |
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Authors: | W Wackernagel |
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Institution: | 1. Department of Medicine Yale University, New Haven, Conn. 06510 USA;2. Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Conn. 06510 USA |
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Abstract: | Genetic transformation of for various chromosomal markers was accomplished by (i) using recipient cells that lack the DNase but were recombination proficient due to or mutations and (ii) treating the recipient cells with CaCl2 at a concentration that facilitates transfection by λ DNA. Cotransformation of three markers () was found to depend on the molecular weight of the transforming DNA. |
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