Oscillations of H+ secretion and electrogenic properties in the isolated frog gastric mucosa

Oscillations of H⁺ secretion rate, active net charge transport measured as short-circuit current and transmucosal electric potential difference with a regular frequency of one period in 45 min appeared spontaneously in the isolated frog gastric mucosa. Similar oscillations were triggered by the addition of histamine at 45-min intervals.The spontaneous oscillations and the continuatino of histamine triggered oscillations after cessation of histamine administration indicates that there was a component of slow inherent rhytmicity in the mucosa. No oscillations were obtained when a small transmucosal gradient of Cl^? was used.With the histamine-triggered oscillations the H⁺ secretion rate was always 180° out of phase with the short-circuit current and the potential difference. This supports the hypothesis of an electrogenic mechanism for active transport of H⁺ in the mucosa that can function at least partially independently of that for Cl^?.