Isolation of cDNA clones encoding two isoforms of nucleoside diphosphate kinase from bovine retina |
| |
Authors: | Karaschuk G Kakuev D |
| |
Institution: | Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow. karas@ibch.siobc.ras.ru |
| |
Abstract: | The cDNA encoding bovine retinal isoforms of nucleoside diphosphate kinase (NDP-kinase, EC 2.7.4.6) has been cloned and sequenced. Based on the partial amino acid sequence of the enzyme determined after trypsin digestion of purified NDP-kinase, primers were synthesized and used to isolate two different cDNA clones encoding the full length of two NDP-kinase isoforms. The nucleotide sequences of these clones contained open reading frames encoding 152-residue polypeptides with calculated molecular masses of 17.262 and 17.299 kDa, similar to that determined for the subunits of purified enzyme (17.5 and 18.5 kDa). The deduced NDP-kinase sequences showed high similarity with the known NDP-kinase sequences from other sources. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|