Substratum adhesion and gliding in a diatom are mediated by extracellular proteoglycans |
| |
Authors: | Jan L Lind Kirsten Heimann Elizabeth A Miller Catherine van Vliet Nicholas J Hoogenraad Richard Wetherbee |
| |
Institution: | (1) School of Botany, University of Melbourne, Parkville, 3052, Australia, AU;(2) Department of Biochemistry, La Trobe University, Bundoora, 3083, Australia, AU |
| |
Abstract: | Diatoms are unicellular microalgae encased in a siliceous cell wall, or frustule. Pennate diatoms, which possess bilateral
symmetry, attach to the substratum at a slit in the frustule called the raphe. These diatoms not only adhere, but glide across
surfaces whilst maintaining their attachment, secreting a sticky mucilage that forms a trail behind the gliding cells. We
have raised monoclonal antibodies to the major cell surface proteoglycans of the marine raphid diatom Stauroneis decipiens Hustedt. The antibody StF.H4 binds to the cell surface, in the raphe and to adhesive trails and inhibits the ability of living
diatoms to adhere to the substratum and to glide. Moreover, StF.H4 binds to a periodate-insensitive epitope on four frustule-associated
proteoglycans (relative molecular masses 87, 112, and >200 kDa). Another monoclonal antibody, StF.D5, binds to a carbohydrate
epitope on the same set of proteoglycans, although the antibody binds only to the outer surface of the frustule and does not
inhibit cell motility and adhesion.
Received: 2 December 1996 / Accepted: 6 March 1997 |
| |
Keywords: | : Adhesion Diatom Extracellular matrix Gliding (diatom) Proteoglycan Stauroneis (extra cellular proteoglycans) |
本文献已被 SpringerLink 等数据库收录! |
|