Detection of inactive precursors of beta-glucanases in Saccharomyces cerevisiae |
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Authors: | L M Hernandez I Olivero G Larriba |
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Affiliation: | Departamento de Microbiologia, Facultad de Ciencias, Universidad de Extremadura, Badajoz, Spain |
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Abstract: | Accumulation and secretion of beta-glucanases have been studied in vivo by using a thermosensitive secretory mutant of Saccharomyces cerevisiae blocked at the endoplasmic reticulum level (sec 18-1). When incubated at the restrictive temperature no accumulation of active glucanases was observed. Following a shift to permissive conditions in the presence of cycloheximide a rise in the internal activity took place. The increase in total glucanase activity was partially due to the activation of an exo-glucanase that hydrolyzes PNPG. It is concluded that glucanases are synthesized in inactive precursor forms and are converted to the active forms in their secretory pathway. |
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Keywords: | Saccharomyces cerevisiae |
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