A microfiltration bioreactor to achieve high cell density in Sulfolobus solfataricus fermentation |
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Authors: | Chiara Schiraldi Francesco Marulli Isabella Di Lernia Angela Martino M De Rosa |
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Institution: | (1) Istituto di Farmacologia e Tossicologia-CRIB, Seconda Università degli Studi di Napoli Facoltà di Medicina, via Costantinopoli 16-80138 Naples, Italy Tel./Fax +39-081-5665866 e-mail: maderosa@ds.unina.it, IT |
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Abstract: | A novel technique is proposed to achieve higher cell yield in extremophile fermentation. Because the accumulation of toxic
compounds is thought to be responsible for low biomass yields, a bioreactor has been designed based on a microfiltration hollow-fiber
module located inside the traditional fermentation vessel. Using the cul-tivation of the thermoacidophilic archeon Sulfolobus solfataricus Gı as a model, a biomass of 35 g l−1 dry weight was obtained which proved greater than that of 2 g l−1 obtained in batch fermentation. The bioreactor was characterized by running several fermentation experiments to check the
high stability of the membrane module to sterilization cycles, high temperatures, and acidic pHs, even for prolonged periods
of time. It was shown that the exhaust medium is unable to sustain growth for the presence of toxic compounds, and ultrafiltration
and ion-exchange techniques were used in all the attempts to regenerate it. The results demonstrated the ability of the method
to lower inhibitor concentrations and prolong the growth phase, thus achieving high cell density. Furthermore, they indicated
that the toxic compounds are ionic species of less than 1kDa.
Received: December 23, 1998 / Accepted: March 18, 1999 |
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Keywords: | Extremophiles Fermentation technology High cell density Microfiltration Sulfolobus solfataricus |
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