P16蛋白和生精细胞凋亡对热压和11酸睾酮诱导的恒河猴无精子症和少精子症中的作用

探讨了P16蛋白和生精细胞凋亡在热压和11酸睾酮诱导恒河猴无精子症和少精子症中作用间的关系。3′末端标记分析（TUNEL）结果显示热应激和超生理剂量睾酮能够诱导生精细胞出现凋亡信号,它分别于处理后第5天和第30天达到最强,免疫组化结果显示,热压或TU主要诱导精原细胞和其它生精细胞以及Sertoli细胞P16的表达。P16蛋白的表达在生精细胞凋亡晚期,即隐睾手术第10天或注射TU第60天后迅速升高并维持在热压或11酸睾酮诱导的早期精母细胞和精子细胞的凋亡和在晚期对精原细胞有丝分裂的抑制,二者共同作用导致热压或TU诱导的恒河猴无精子症和少精子症。

ROLE OF P16 PROTEIN AND GERM CELL APOPTOSIS IN RHESUS MONKEY AZOOSPERMIA OR OLIGOZOOSPERMIA INDUCED BY HEAT STRESS OR TESTOSTERONE UNDECANOATE

We investigated the functional relationship between the role of P16 protein and germ cell apoptosis in heat stress or TU induced azoospermia or oligo zoospermia.The TUNEL result showed that the apoptotic signals after treatment increased and the strongest apoptotic signals appeared in the germ cells of the cryptorchid testes on day 5 or in the TU treated monkey testes on day 30.The immunohistochemical result showed that both heat stress and TU induced expression of P16 protein mainly in spermatogonia,and also in other type of germ cells and sertoli cell at the later stage of germ cell apoptosis,namely on day 10 after operation or on day 60 after TU injection respectively, suggesting that P16 protein may inhibit the spermatogonia mitosis at the later stage of the germ cell apoptosis.The above results indicated that both heat stress and TU treatment were capable of inducing germ cell apoptosis at the early stage on one hand,and inhibiting spermatogonia mitosis at the later stage in the testis on the other hand,which might result in azoospermia or oligozoospermia.