Clearing Specimens for the Demonstration of Bone

A brief review is given of some of the older technics of clearing and staining various types of animals, in such a way that the bones are clearly visible in the surrounding tissues. The authors have modified some of these earlier practices. Particularly good results were obtained by combining the Schultze technic with that of Lundvall. Embryos are fixed in 95% alcohol, treated with 1% KOH, stained in 1:10,000 alizarin Red S, cleared in gylcerin, dehydrated with alcohol and further cleared in toluol, toluol saturated with naphthalene, and anise oil saturated with naphthalene. Details of the technic are presented in the text