High-performance liquid chromatography determination of residue levels on chicken carcasses treated with cetylpyridinium chloride |
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Authors: | Xiang Zhou Andronica Handie Hamid Salari E Kim Fifer Philip J Breen Cesar M Compadre |
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Institution: | Food Safety Consortium Group and Department of Pharmaceutical Sciences, University of Arkansas for Medical Sciences, Slot 522, 4301 W. Markham, Little Rock, AR 72205, USA |
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Abstract: | Cetylpyridinium chloride (CPC) has been found to be effective in reducing contamination of chicken carcasses from a variety of microorganisms, including Escherichia coli O157:H7, Salmonella typhimurium, Campylobacter jejuni, Aeromonas hydrophila, Listeria monocytogenes, and Staphylococcus aureus. A procedure has been developed to determine residue levels on chicken carcasses after CPC treatment. For the analysis, chicken carcasses were extracted with 95% ethanol. The CPC concentration in the extract was measured by high-performance liquid chromatography (HPLC) with ultraviolet detection using dodecylpyridinium chloride (DPC) as an internal standard. The method was validated in the concentration range of 3–200 μg/ml CPC in ethanolic extract. This assay is rapid, precise, and accurate. |
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Keywords: | Cetylpyridinium chloride |
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