Assay method for the perfluorooctyl bromide (perflubron) in rat blood by gas chromatography–mass spectrometry |
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Authors: | Michel Audran Marie-Pierre Krafft Jacques De Ceaurriz Jean-Charles Mathurin Marie-Thrse Sicart Bndicte Marion Fabien Fabre Franoise Bressolle |
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Institution: | a Département de Biophysique, Faculté de Pharmacie, Université Montpellier I, Montpellier, France;b Institut Charles Sadron (CNRS), Strasbourg, France;c Laboratoire National de Dépistage du Dopage, Chatennay Malabry, France;d Laboratoire de Pharmacocinétique Clinique, Faculté de Pharmacie, Université Montpellier I, Montpellier, France |
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Abstract: | This paper describes a GC–MS method (SIM mode) for the analysis of perfluorooctyl bromide (perflubron, I) in rat blood. The chromatographic separation was performed by injection in the split mode using a CP-select 624 CB capillary column. Following destruction of the emulsion by addition of ethanol, the analytical procedure involves a liquid–liquid extraction with 1,1,2-trichlorotrifluoroethane. The bis(F-butyl)ethene (II) was used as internal standard. Observed retention times were 3.22 min for I and 2.32 min for II. Two calibration curves were used; linear detection responses were obtained for concentrations ranging from 0.009 to 0.9 mg/ml and from 0.9 to 13.5 mg/ml. The extraction efficiency averaged 50% for I and 93% for II. Precision ranged from 0.7 to 14%, and accuracy was between 91 and 109%. The limit of quantification was 9 μg/ml. The method validation results indicate that the performance characteristics of the method fulfilled the requirements for assay method for use in pharmacokinetic studies. |
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Keywords: | Perfluorooctyl bromide Perflubron |
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