Isolation and Characterisation of a High-Efficiency Desaturase and Elongases from Microalgae for Transgenic LC-PUFA Production |
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| Authors: | James R. Petrie Qing Liu Anne M. Mackenzie Pushkar Shrestha Maged P. Mansour Stan S. Robert Dion F. Frampton Susan I. Blackburn Peter D. Nichols Surinder P. Singh |
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| Affiliation: | (1) Food Futures National Research Flagship, CSIRO Plant Industry, P. O. Box 1600, Canberra, ACT, 2601, Australia;(2) Food Futures National Research Flagship, CSIRO Marine and Atmospheric Research, P. O. Box 1538, Hobart, TAS, 7000, Australia |
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| Abstract: | The production of long-chain polyunsaturated fatty acids from precursor molecules linoleic acid (LA; 18:2ω6) and α-linolenic acid (ALA; 18:3ω3) is catalysed by sequential desaturase and elongase reactions. We report the isolation of a front-end Δ6-desaturase gene from the microalgae Ostreococcus lucimarinus and two elongase genes, a Δ6-elongase and a Δ5-elongase, from the microalga Pyramimonas cordata. These enzymes efficiently convert their respective substrates when transformed in yeast (39–75% conversion for ω3 substrate fatty acids), and the Δ5-elongase in particular displays higher elongation efficiency (75% for conversion of eicosapentaenoic acid (20:5ω3) to docosapentaenoic acid (22:5ω3)) than previously reported genes. In addition, the Δ6-desaturase is homologous with acyl-CoA desaturases and shows a strong preference for the ω3 substrate ALA. |
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