Edelfosine-induced metabolic changes in cancer cells that precede the overproduction of reactive oxygen species and apoptosis |
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| Authors: | Vitaly A Selivanov Pedro Vizán Faustino Mollinedo Teresa WM Fan Paul WN Lee Marta Cascante |
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| Affiliation: | 1.Department of Biochemistry and Molecular Biology,Faculty of Biology, Institute of Biomedicine of University of Barcelona (IBUB) and IDIBAPS, Unit Associated with CSIC,Barcelona,Spain;2.A.N.Belozersky Institute of Physico-Chemical Biology, Moscow State University,Moscow,Russia;3.Centro de Investigación del Cáncer, Instituto de Biología Molecular y Celular del Cáncer, Consejo Superior de Investigaciones Científicas (C.S.I.C.)-Universidad de Salamanca,Salamanca,Spain;4.Department of Chemistry,Center for Regulatory and Environmental Analytical Metabolomics, University of Louisville,Louisville,USA;5.Department of Medicine,Structural Biology Program, James Graham Brown Cancer Center, University of Louisville,Louisville,USA;6.Department of Pediatrics,Research and Education Institute, Harbor-UCLA Medical Center,Torrance,USA;7.Laboratory of Developmental Signalling, Cancer Research UK London Research Institute,London,UK |
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| Abstract: | Background Metabolic flux profiling based on the analysis of distribution of stable isotope tracer in metabolites is an important method widely used in cancer research to understand the regulation of cell metabolism and elaborate new therapeutic strategies. Recently, we developed software Isodyn, which extends the methodology of kinetic modeling to the analysis of isotopic isomer distribution for the evaluation of cellular metabolic flux profile under relevant conditions. This tool can be applied to reveal the metabolic effect of proapoptotic drug edelfosine in leukemia Jurkat cell line, uncovering the mechanisms of induction of apoptosis in cancer cells. |
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