应用酶联免疫吸附试验检测不同类型乙型肝炎中激活补体类HBsAg循环免疫复合物

本文以抗人C_(?)的羊IgG为包被抗体,以HRP-HBs抗体为指示抗体,建立了可检测激活补体类HBsAg循环免疫复合物(HBsAg/C3-CIC)的C_3捕捉法酶联免疫吸附试验。检测了236例六种类型临床诊断为乙型肝炎的病人血清标本,其阳性率分别为:无症状携带者(ASC)12.9％(4/31),急性肝炎(AH)36.7％(22/60),慢性迁延性肝炎(CPH)33.3％(7/21),慢性活动性肝炎(CAH)59.6％(34/57),重型肝炎(SH)77.8％(14/18),肝炎后肝硬化(PLC)67.3％(33/49),阳性率与肝损严重程度明显相关(P<0.01)。认为HBs-Ag/C3-CIC可能在乙型肝炎病毒引起的慢性活动性肝炎、重型肝炎和肝炎后肝硬化的发病过程中起重要作用,并可作为乙型肝炎的诊断、临床分型和预后判断的指标之一。

DETECTION OF CIRCULATING HBsAg IMMUNE COMPLEXES ACTIVATING COMPLEMENT BY ELISA IN DIFFERENT CLINICAL TYPES OF HEPATITIS B INFECTION

By an antibody capture-ELISA method, the circulating HBsAg immune complexes activating complement ( HBsAg/C3-CIC ) of 236 cases of hepatitis B and HBsAg carrier were examined. The positive rates of HBsAg/C3 -CIC in HBsAg carrier, acute hepatitis B, chronic persistent hepatitis B, chronic active hepatitis B, severe hepatitis B, cirrhosis after hepatitis B were 12.9% (4/31 ) ,36.7% (22/60), 33.3% ( 7/21 ), 59.6% ( 34/57 ), 77.8% ( 14/18 ) and 67.35 ( 33/49 ) , respectively. The ratss of HBsAg-CIC were positively to related with the degree of damaged liver in different types of hepatitis B( P<0.01 ) . It is concluded that HBsAg/C3-CIC may play an important role in the mechanism of liver damage in chronic active hepatitis B, severe hepatitis B and cirrhosis after hepatitis B, and may be one of valuable marker for the diagnosis and prognosis of hepatitis B.