Transductional analysis of chromosome replication time |
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Authors: | Mark C. Hanks and Millicent Masters |
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Affiliation: | (1) Department of Molecular Biology, King's Buildings, Mayfield Road, EH9 3JR Edinburgh, UK;(2) Present address: Poultry Research Unit, Roslin, Midlothian, UK |
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Abstract: | Summary Following transduction of exponentially growing cultures of Escherichia coli with phage P1, cells with recombinant phenotype begin to increase in number after an initial lag of about one generation time. We show that transductants for markers located at different positions on the chromosome begin to increse at different times, in reverse order to that in which they are replicated. The period over which this happens is equal in duration to the time taken to replicate the chromosome and we have used this relationship to calculate the C-period of E. coli K12 growing at 30°C. We exclude transduction-induced filamentation as the cause of the initial lag and suggest that the lag may result from the way in which donor DNA is inherited. |
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Keywords: | Transduction DNA replication Recombination Bacteriophage P1 |
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