Electron microscopy of the actin-myosin head complex in the presence of ATP. |
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Authors: | L L Frado R Craig |
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Institution: | Department of Cell Biology, University of Massachusetts Medical School, Worcester 01655. |
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Abstract: | The structure of the actin-myosin head complex during the ATPase cycle has been studied by electron microscopy of negatively stained acto-heavy-meromyosin. In the absence of ATP, heavy meromyosin molecules generally showed a regular, angled appearance, with both heads attached to the actin filament. In the presence of ATP, attached molecules showed a less ordered structure, often with only one head attached. We conclude that configurations other than the rigor structure occur during the actomyosin cross-bridge cycle. |
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