A UV Tolerant Mutant of Bacillus thuringiensis subsp. kurstaki Producing Melanin |
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| Authors: | Deepak Saxena Eitan Ben-Dov Robert Manasherob Ze'ev Barak Sammy Boussiba Arieh Zaritsky |
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| Institution: | (1) Department of Life Sciences, Ben-Gurion University of the Negev, P.O. Box 653, Be'er-Sheva 84105, Israel, IL;(2) The Research Institute, The College of Judea and Samaria, Ariel 44837, Israel, IL;(3) Microalgal Biotechnology Laboratory, Blaustein Institute for Desert Research, Ben-Gurion University at Sede-Boker, 84990, Israel, IL |
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| Abstract: | A UV-resistant mutant (Bt-m) of Bacillus thuringiensis subsp. kurstaki, producing a dark brown pigment, identified as melanin, was studied. Bt-m had higher larvicidity against Heliothis armigera than its parent. Survival of Bt-m spores and their insecticidal activity to irradiation at 254 nm and 366 nm were higher than those of the parent. The only
toxic polypeptide produced by Bt-m was Cry1Ac (130 kDa); it lost cry1Aa, cry2Aa, and cry2Ab.
Received: 2 April 2001 / Accepted: 14 May 2001 |
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