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Intraerythrocytic administration of a synthetic Plasmodium antigen elicits antibody response in mice, without carrier molecules or adjuvants |
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| Authors: | Fulvio Esposito Anna Maria Santroni Annette Habluetzel Vincenzo Grelloni Antonello Pessi Stefania Lombardi Giancarlo Falcioni and Maurizio Brunori |
| Institution: | * Dipartimento di Biologia Molecolare, Cellulare e Animale, Universita' di Camerino, via Camerini 2, 62032, Camerino (MC, Italy † Istituto Zooprofilattico Sperimentale, Perugia, Italy ‡ Sclavo SpA, Monterotondo, Roma, Italy § Istituto di Parassitologia, Universita' “La Sapienza”, Roma, Italy | Dipartimento di Scienze Biochimiche Universita' “La Sapienza” & Centro di Biologia Molecolare del CNR, Roma, Italy |
| Abstract: | The synthetic peptide (NANP)20, reproducing the tandem-repeated epitope of the circumsporozoite protein of Plasmodium (Laverania) falciparum, was entrapped into murine, autologous erythrocytes by a hypotonic dialysis method. Mice immunized intravenously with minute amounts of encapsulated peptide produced considerable antibody titres. This result indicates that intraerythrocytic antigen administration may have a potential as an immunization system for humans, since it dispenses with adjuvants and carrier molecules. |
| Keywords: | Plasmodium malaria vaccine immunization antibodies antigen synthetic peptides circumsporozoite protein erythrocytes |
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