Molecular characterization of Pisolithus spp. isolates by rDNA PCR-RFLP |
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| Authors: | Eliane A. Gomes Everaldo G. de Barros Maria Catarina M. Kasuya Elza F. Araújo |
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| Affiliation: | Departamento de Microbiologia/BIOAGRO, Universidade Federal de Vi?osa, Vi?osa, MG, 36571-000, Brazil, Fax: +55-31-899-2573, e-mail: ezfa@mail.ufv.br, BR
Departamento de Biologia Geral/BIOAGRO, Universidade Federal de Vi?osa, Vi?osa, MG, 36571-000, Brazil, BR
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| Abstract: | Variation within ribosomal DNA (rDNA) genes of 19 isolates of Pisolithus from different geographic origins and hosts was examined by polymerase chain reaction (PCR) coupled with restriction fragment length polymorphism (RFLP) analysis. The primers utilized amplify rDNA regions in a wide range of fungi. One amplified region includes the internal transcribed spacer (ITS), which has a low degree of conservation. The ITS amplification products (640–750 bp) were digested with a variety of restriction endonucleases. Cluster analysis based on the restriction fragments grouped the isolates into three distinct groups: group I contained isolates collected in the northern hemisphere, except Pt 1, group II contained those collected in Brazil and group III contained isolate Pt 1. Additional analysis of other rDNA regions, IGS, 17 S and 25 S rDNA, resulted in similar groups. The data suggest that the taxonomy and systematics of this ectomycorrhizal fungus should be revised. Accepted: 16 September 1998 |
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| Keywords: | DNA polymorphism Ectomycorrhizal fungi PCR-RFLP Pisolithus spp. Ribosomal DNA |
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