Cytokinins of dryZea mays seed: Quantification by radioimmunoassay and gas chromatography-mass spectrometry |
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| Authors: | C H Hocart J Badenoch-Jones C W Parker D S Letham R E Summons |
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| Institution: | (1) Research School of Biological Sciences, Australian National University, ACT 2601 Canberra City, Australia;(2) Division of Continental Geology, Bureau of Mineral Resources, ACT 2601 Canberra, Australia;(3) Present address: Institut für Organische Chemie, Universität Bern, CH-3012 Bern, Switzerland |
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| Abstract: | The endogenous cytokinins present in dryZea mays seed were determined using both radioimmunoassay and gas chromatography—mass spectrometry. Similar values for bases and ribosides were obtained by the two methods. The cytokinins present in embryo and endosperm were estimated separately using radioimmunoassay; similar levels of cytokinins were found in these two tissues. The major cytokinins detected on a whole-seed basis were dihydrozeatin riboside, O-glucosyldihydrozeatin riboside, zeatin 9-glucoside, zeatin, and the nucleotides of zeatin, dihydrozeatin, and isopentenyladenine. Cytokinin levels in the mature dry seed were considerably lower than cytokinin levels published in the literature for immature seed. Unexpected activity in the radioimmunoassays was detected in the wash from the DEAE cellulose column chromatography step. The compound(s) responsible for this activity did not have the solvent partitioning characteristics of a cytokinin base or riboside. They eluted as a single fraction following high-performance liquid chromatography on a Zorbax C8 column; this fraction showed no activity in theAmaranthus bioassay for cytokinins, but inhibited the activity of authentic zeatin riboside present at an optimal concentration. |
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