Interactions between mgr, asp, and polo: asp function modulated by polo and needed to maintain the poles of monopolar and bipolar spindles |
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Authors: | Cayetano Gonzalez Claudio E Sunkel David M Glover |
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Institution: | (1) Cell Biology Programme, EMBL, Heidelberg, Germany, DE;(2) Instituto de Biologia Molecular e Celular, Universidade do Porto, Rua do Campo Alegre 823, P-4150 Porto, Portugal, PT;(3) CRC Cell Cycle Genetics Group, Cancer Research Campaign Laboratories, Department of Anatomy and Physiology, The University of Dundee, Dundee DD1 4HN, UK, GB |
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Abstract: | We describe genetic interactions between mutations in mgr, asp, and polo, genes required for the correct behaviour of the spindle poles in Drosophila. The phenotype of a polo
1
mgr double mutant is more similar to mgr than polo
1
, but the frequency of circular monopolar figures (CMFs) seen with either mutant alone is additive, suggesting that the two
gene products are required for independent functions in the formation of bipolar spindles. The asp
E3
mgr double mutant arrests much earlier in development than either mutant alone, indicative of a strong block to cell proliferation.
We discuss whether the lack of microtubular structures in these cells reflects an extended mitotic arrest, or if it is a more
direct consequence of the double mutant combination. A polo
1
asp
E3
double mutant shows a dramatic synergistic increase in mitotic frequency. The loss of CMFs normally associated with the polo
1
phenotype suggests that the Asp microtubule-associated protein is required to maintain the structure of spindle poles. We
speculate that Asp protein might be a substrate for the serine-threonine protein kinase encoded by polo.
Received: 8 August 1998 / Accepted: 13 September 1998 |
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