Identification of a rabbit B cell alloantigen with an antiserum produced by alloimmunization with thymus cells. |
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Authors: | B S Wilson A J Strelkauskas S Dray |
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Institution: | Department of Microbiology, University of Illinois at the Medical Center, Chicago, Illinois 60680 USA |
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Abstract: | Alloimmunization with rabbit thymus cells resulted in an antiserum (anti-Rly) which was shown to react with rabbit lymphocytes by an indirect rosette technique. The titration curve obtained with dilutions of anti-Rly antiserum on lymph node cells revealed two plateaus indicating that the antiserum was multispecific; at low dilutions of antiserum, within the first plateau, both B and T cells were rosetted whereas at high dilutions, within the second plateau, only B cells were rosetted. The antigen detected at high dilution was designated LB-1 (lymphocyte B cell alloantigen 1). The evidence that the cells identified within the second plateau are B cells is as follows: 1) simultaneous enumeration of LB-1+ and Ig+ (B) cells by use of distinguishable erythrocytes (sheep and human) as indicator cells revealed that of the 53% rosettes observed, essentially all (51%) were mixed rosettes containing both erythrocytes whereas simultaneous enumeration of LB-1+ and T+ cells (identified by anti-T cell antiserum) showed essentially no mixed rosettes (less than 2%); 2) approximately 80% of purified Ig+ (B) cells were identified as LB-1+ cells whereas essentially no (< 1%) purified T cells could be detected as LB-1+; 3) the percentages of LB-1+ cells and Ig+ cells were both reciprocal to the precentages of T+ cells identified in various lymphoid organs except for bone marrow; 4) the removal of LB-1+ cells from spleen cells of rabbits immunized with sheep red blood cells resulted in a depletion (42–71%) of direct plaque forming cells (PFC). Since the percentages of bone marrow cells rosetted using anti-LB-1 antiserum (approximately 70%) was much greater than the percentage rosetted using anti-Ig (approximately 10%), it appears that the anti-LB-1 antiserum is not directed against an Ig allotype. The titration curves of the anti-Rly antiserum on peripheral blood lymphocytes of a large rabbit family suggested that the LB-1 antigen on B cells is an alloantigen probably inherited in simple Mendelian fashion. Adsorption studies indicated that the LB-1 antigen on B cells is not detectable on brain, liver, kidney or erythrocytes. |
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Keywords: | Correspondence and reprint requests to: Dr Sheldon Dray Professor and Head Department of Microbiology University of Illinois at the Medical Center P O Box 6998 Chicago Illinois 60680 |
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